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Immediately before rodents were inoculated with virus, a baseline serum sample was taken from each rodent for subsequent antibody assays.
Having higher HI titers in the baseline serum sample was protective (OR 0.5 per unit of increase, 95% CI 0.3 1.0; p = 0.05).
For the analysis of serologically-confirmed infections, we excluded vaccinated individuals where the baseline serum sample was taken fewer than 14 days after vaccination, or where a later second sample was not obtainable (see recruitment above).
Miller et al. reported that 89.1% of participants with pandemic (H1N1) 2009 had an antibody titer of >32 three weeks after infection, although a baseline serum sample was not taken; therefore, seroconversion could not be demonstrated (17 ).
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From January 25 to 27, 2004, a nasal swab specimen and baseline serum sample were collected from healthcare workers at the hospital; each worker also completed a questionnaire.
The median time between last exposure and collection of the nasal swab and the baseline serum samples was 7 days (range 2 12 days).
Baseline serum samples were available for 85 (77%) of the women.
For the MBL level analysis baseline serum samples were available from 246 of 258 patients.
Baseline serum samples were used to avoid a possible influence of treatment on antibody levels.
The baseline serum samples were all negative at a dilution of 1 64.
Baseline serum samples were assayed for RF was using a latex method and a titre of ⩾1 40 was regarded as positive.
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