Exact(2)
Baseline sequences from the United States clustered predominantly into clade 1 (68%, 129/190 sequences), while baseline sequences from the European Union clustered predominantly into clade 2 (73%, 27/37 sequences).
The GT1b strain Con1 and GT1c strain HC-G9 reference sequences each sorted as separate groups, and GT1a baseline sequences from the AVIATOR study sorted into 2 distinct clades, with the GT1a H77 reference sequence sorting to clade 1.
Similar(56)
Phylogenetic analyses were conducted using baseline sequences from GT1a- and GT1b-infected patients to determine the genetic relatedness of the DAA target sequences according to geographic region.
Given the heterogeneity of the HCV genome, phylogenetic analyses were conducted on NS3, NS5A, and NS5B baseline sequences from patients infected with GT1a and GT1b to compare the genetic relatedness of the sequences according to geographic region.
Baseline virus sequences from the other eight HLA-A2-positive patients all carried glycine in position 83, which in seven of the patients was linked to a leucine, phenylalanine or arginine in position 85 (Table 4).
Phylogenetic analyses were conducted to ensure proper assignment of HCV GT4 subtype using NS5B (a 329-nucleotide region [ 21]) and full-length NS3/4A and NS5A gene sequences from the baseline samples of HCV GT4-infected patients.
In order to establish a phylogenetic analysis, the entire genome from the viruses should ideally be sequenced (Neuraminidase gene contains approximately 1,413 bp); the partial NA sequences generated by our group (729 bp) are of importance as they could provide a baseline of NA sequences from where the pandemic emerged.
In subject 18, the three most common baseline forms declined from a total of 86% to just 1.5% of the V3 loop population, while a CXCR4-using form present at 0.8% of baseline sequences rose to 85%.
The sequences from the Vitis vinifera (cv.
Second, sequencing technologies offer several ways to reduce the baseline sequencing error rate.
The standard curve with a dilution range of 102 107 copies/mL was generated using RNA synthesized from a plasmid that contained the dominant subject-specific V3 loop sequence from the baseline (week 0) sample (TOPO-TA Kit, [Invitrogen] and Megascript T7 High Yield Transcription Kit [Ambion]).
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