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Baseline samples were fixed directly after preparation of fresh tissue and represent the status of the vessel prior to organ culture.
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The samples were fixed in 4% formaldehyde.
Samples were fixed in 4% paraformaldehyde overnight.
Samples were fixed in 5% formalin solution.
Lung samples were fixed in 10% buffered formalin.
Samples were fixed with 4% paraformaldehyde (PFA) in relaxing solution.
All samples were fixed in 4% buffered formalin.
Samples were fixed to the membrane by UV-crosslinking (70 mJ/cm2).
Embryonic brain samples were fixed overnight in 4% paraformaldehyde (PFA).
After treatment, samples were fixed with 10% formaldehyde.
Tissue and tumor samples were fixed in formalin.
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