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The concentration of plant-available elements was determined after Mehlich III extraction (Mehlich 1984) followed by ICP-AES analysis on baseline samples collected for season 1.
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Two pre-treatment baseline samples (collected on separate days) were used to establish a mean pre-treatment value.
Table 1 shows demographis characteristics of the samples collected for the community, healthcare workers and baseline study.
Dialysate samples were collected at 20 min intervals through previously implanted probes; baseline samples were collected for 40 min and then rats were administered varenicline (1.0 mg kg−1, s.c).
The blood samples and baseline and endpoint samples collected were analyzed for SCFA.
The models were also adjusted for BMI, age and time when baseline samples were collected to account for circadian variation.
At baseline, blood samples were collected for determination of lipids, high sensitivity C-reactive protein (hsCRP), HbA1c, hyaluronan and hyaluronidase.
Briefly, after an overnight fast, at 08 00 h baseline blood samples were collected for assessment of leptin, interleukin-6, insulin and cortisol.
Before meal ingestion, three baseline blood samples were collected for fasting glucose, insulin, glucagon, and IAPP concentrations and [1-C]glucose enrichments.
For patients in the LF group, the blood samples at baseline were collected for measuring fasting blood glucose and FFA0 levels 15 min before intravenous injection of 18F-FDG (370 MBq).
The baseline spectra collected for the samples with TTX also contained the toxin.
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