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The chance of observing seroconversion was maximized at 82% (95% CI 69% to 91%) when the analysis was restricted to the 45 participants where the baseline sample was collected earlier (before onset or less than 5 days from illness onset), and the follow-up sample collected later (15 days or more days after illness onset).
A baseline sample was collected 20 hours prior to drug administration for all routes.
A baseline sample was collected in early August 2009, a time frame which more or less coincided with the early phase of the 2009 influenza A (H1N1) epidemic in Taiwan.
For every experiment, one baseline sample was obtained from t = −30 min to t = 0 min. The first baseline sample was collected approximately 2.5 h after the lights turned off.
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Baseline samples were collected 15 min before CCI (under 1 2% isoflurane), 5 min pre-CCI, 10 min post-CCI, and 60 min post-CCI.
After 1.5 h of stabilization, baseline samples were collected into 7.5 μl 0.02 M formic acid (FA) at 15 min intervals for 2 h.
After 20 minutes for equilibration, a series of five 20-min baseline samples were collected.
Dialysis perfusion was started one hour later, at which time the mice were left undisturbed for approximately 2 h before baseline samples were collected.
Also, the relatively small numbers of opportunistic diagnostic sera in the baseline samples were collected without randomization and with no information on seasonal influenza vaccination and other important determinants.
After stable baseline levels of 5-HT and DA were obtained, six baseline samples were collected.
An antecubital intravenous line was inserted, and baseline samples were collected.
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