Comparison of baseline gene expression profiles in PBMC identified differentially expressed genes in the two groups.
Patients with abdominal sepsis had significantly more variability in the baseline gene expression compared with the other sepsis patients (SD 4.1 ± 0.8 vs. 2.8 ± 0.8, p = 0.03).
Our experience is that a fold change of 1.5 can be used with an acceptable sensitivity (especially in combination with the FDR-adjusted p value threshold of <0.01) and may also yield an understanding of potential downstream effects which are biologically relevant compared with the normal baseline gene expression [40, 41].
C57BL6 mice were used for the baseline gene expression.
Candesartan and aliskiren did not affect baseline gene expression in the non-ischemic contralateral hemisphere.
As shown in Figure 3, mycoplasma affects baseline gene expression for TLR4, IL1R2, IKB1Z, and IL10.
However, it appears to have a significant role in regulating baseline gene expression.
In a previous paper we showed that baseline gene expression could predict future MODS and other clinical events [5].
In this independent study group we confirmed the negative correlation between baseline gene expression level and treatment induced biological response (p<0.0085 and R = −0.4719).
Comparing baseline gene expression levels and biological response using the average of these 15 genes revealed a significant negative correlation (R = −0.7208; p = 0.0016) (figure 2B).
Proactive and reactive fish differed in baseline gene expression and also showed diametrically opposite responses to the challenge for 80% of the genes investigated.
