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The baseline expression levels of SOD2 and PGC-1α were reduced in fibroblasts from FRDA patients when compared to healthy controls cells (Fig. 1A).
The results described above could point towards a method to predict responsiveness to IFNß therapy based on baseline expression levels of IFN-induced genes.
This indicates that the baseline expression levels of the selected set of 15 IFN-induced genes can be used as a predictive marker for the responsiveness to IFNß treatment.
Having established baseline expression levels of DSPP in two OSCC cell lines, we proceeded to investigate how stable DSPP-silencing via lentiviral-mediated small hairpin RNA (shRNA) interference affects the tumorigenic profiles of OSC2 cells.
Previously, we demonstrated significant differences in the expression of type I IFN-induced genes between untreated RRMS patients.[14] Here we investigated whether there is a relationship between the differential in vivo responsiveness to IFNß and baseline expression levels of IFN-induced genes.
At baseline, expression levels of the miR-874 were negative in all cell lines.
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We hypothesized that baseline expression level of these genes influences subsequent IFNß signaling upon treatment.
However, the baseline expression level of each of the two genes varied from batch to batch (figure 3B,C).
This variability did not correlated with the age at which we transfected retinas or the baseline expression level of the indicators.
The effect of SYT-SSX1 was inversely proportional to the baseline expression level of the two genes prior to infection: the lower their baseline expression level, the stronger was the induction by SYT-SSX.
Although the baseline expression level of CXCL10 varied in individual patients, CXCL10 expression in RA synoviocytes was increased by TNF-α.
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