After 40 min of rest, a baseline blood sample was taken and a bolus injection of semisynthetic human insulin was given over 10 min, followed by continuous infusion (56 mU × min−1 × m2 × body surface area−1) for 110 min.
Intervention After an initial clinic visit, during which a baseline blood sample was collected for measurement of plasma folate and total homocysteine concentrations, subjects participated in focused dietary recall interviews by telephone and/or Internet (e-mail).
After a baseline blood sample was obtained, a second catheter was inserted in the contra-lateral arm for the primed-constant infusion of L-[ring-13C6]phenylalanine (prime: 2 µmol kg−1, 0.05 µmol kg−1 min−1; Cambridge Isotope Laboratories, Woburn, MA, USA).
For dissociation kinetics, a separate set of mice were instrumented and ventilated with 500 ppm CO for 80 minutes, following which a baseline blood sample was taken, the inspired gas changed to air, and ventilation continued for a further 80 minutes with blood sampling every 20 minutes.
A baseline blood sample was taken after overnight fasting.
After an overnight fast, a baseline blood sample was obtained.
The baseline blood sample is preferably taken before surgery or start of treatment.
Each participant's baseline blood sample is collected as part of their ascertainment and upstream recruitment into the ACRB according to the CoBRA procedures.
The baseline blood samples were analyzed with a multiplexed dPCR assay validated for ultra-sensitive detection of WT and hotspot ESR1 mutations (D538G, Y537S, Y537C, and Y537N, Supplementary Fig. S2a-d).
Baseline blood samples were obtained before surgery (T0), at admission to the ICU (T1) and the day following admission to the ICU (T2).
