Here is Basel's classy red Rathaus (City Hall), with its distinctive tall tower decorated with Swiss flags; take a look inside to see the artfully painted walls, some of which were completed in 1608 by German Renaissance painter Hans Bock who resided in Basel.
After collagenase neutralisation, the cells were washed by Hank's balanced saline solution (Lonza, Basel, Switzerland), and red blood cells were lysed with Red Blood cell Lysis Solution (Promega Corp).
HMECs were cultured in human mammosphere medium (HMM): Hepes-buffered DMEM/F12 without phenol red (Gibco, Basel, Switzerland) supplemented with 20 ng/ml EGF (Invitrogen, Basel, Switzerland), 1 × B-27 (Gibco) and 1 nM 17-β-oestradiol (Sigma, Buchs, Switzerland).
Apoptotic cells were identified using an In Situ Cell Death Detection Kit, TMR red (Roche Applied Science, Basel, Switzerland) that stains apoptotic cells red.
TUNEL assay was performed with the In Situ Cell Death Detection Kit, TMR red (Roche, Basel, Switzerland), according to the manufacturer's instructions.
Cells were washed and cultured in RPMI 1640 medium w/o phenol red (Lonza, Basel, Switserland), supplemented with 5% FCS (Bodinco, Alkmaar, The Netherlands), 100 U/ml penicillin, 100 µg/ml streptomycin, 2 mM L-Glutamine, 50 µM 2-ME, 20 µg/ml human apo-transferrin ((Sigma-Aldrich), depleted for human IgG with protein G sepharose (Amersham, Uppsala, Sweden)) and 10 µg/ml gentamycin.
The TUNEL assay was performed in 30- μm cryostat tissue sections using the in situ Apoptosis Detection Kit with TMR red (Roche, Basel, Switzerland, Cat. no. 12156792910).
Cell death detected in cryo-sectioned electroporated embryos by terminal transferase UTP nicked end labelling (TUNEL) using In Situ Cell Death Detection Kit, TMR red (Roche, Basel Switzerland), according to manufacturer's protocol.
TUNEL assay was performed on muscle sections from sham and Cll-treated mice 3 days after CTX injection using the In Situ Cell Death Detection Kit, TMR red (Roche, Basel, Switzerland), as described by the manufacturer.
JS There was no hard-luck story for Liverpool against FC Basel, despite Lazar Markovic's controversial red card and the late siege on the Swiss goal that was sparked by Steven Gerrard's trademark heroics.
