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Poxvirus based vectors have been established as a potent system for the development of candidate recombinant vaccines for many viral diseases [26], [27].
To further expand the applicability of the siRNA approach, recombinant retro- and adenoviral based vectors have been designed [ 3, 4].
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Now that the essential base vectors have been identified we can start to simplify the structure with a view to understanding the key components in its construction.
Alphavirus-based vectors have been engineered for in vitro and in vivo expression of heterelogous genes.
Several positive selection system-based vectors have been considered efficient tools to date for simplifying in vitro DNA recombination procedures (Liu et al. 2011).
The viral-based vectors have been developed as an alternative strategy of gene therapy.
Cationic liposome (lipoplex) and polymer (polyplex -based vectors have been developolyplex -basedl gene delivectors
Consequently, strategies to reduce the kidney uptake of radiolabeled protein- or peptide-based vectors have been investigated intensively.
Classical Moloney murine leukemia virus (MLV) based retroviral vectors have been used for over 20 years to transfer genes into dividing cells.
Vaccination of horses with recombinant MVA based vectors has also been shown to be an effective means of inducing protective immunity to influenza virus infection [32], [45].
Several vaccine strategies based on viral vectors have been evaluated in preclinical and clinical trials, with largely disappointing results.
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