All oligonucleotide primer sets were designed based upon the published mRNA sequence.
Based upon the published literature, few valid inferences can be drawn about the relative merits of one intervention over another.
These assessments were made from the then currently available literature and presented potential biases based upon the published data.
The pathways and networks were constructed based upon the published literature, Ingenuity Pathways Analysis (Ingenuity Systems, Inc), and PathwayArchitect 2.0.1 (Stratagene, La Jolla, CA).
Discharges to rehabilitation units were costed based upon the published literature, which suggests an average length of stay of 33 days at a cost of £2,758 (Table 1) [ 17].
Based upon the published data, from 1988 to date, generated from animal PBVs across the globe, this review provides information and discussion with respect to genetic analysis as well as evolution of PBVs of animal origin in relation to human strains.
Based upon the published literature, particularly the large collection of in vitro work demonstrating improved cellular response, there is little doubt that the growth factor milieu contained within PRP has the potential to be highly beneficial to bone regeneration.
As there is no experimentally determined 3-dimensional structure for the B. mallei protein, the GroEL tertiary structure was predicted (100% confidence) using Phyre2 [ 17], based upon the published crystal structure of GroEL from E. coli with a 76% sequence identity [ 18].
For the methylation analysis, the primers for the three Igf2/H19 DMRs were designed and based upon previously published sequences.
We performed multivariable logistic regression to determine predictors of willingness to participate, with predictor variables chosen a priori based upon previously published data or suspected influence on willingness to participate in research.
