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Based upon the sequenced gene cluster and the recombinant expression performed here, it is clear that the combination of PKS-NRPS and auxiliary ER lead to the formation of 12. Unlike our findings here, the wild-type fungus did not produce any cis-decalin product, such as 11.
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Rodent oligonucleotide arrays are based upon the sequence of the B6 mouse or Brown-Norway (BN) rat strains.
Because our probes were designed based upon the sequence of the B73 reference genome [21], each exhibits a perfect match to B73.
The medians were calculated based upon the sequences showing the highest alignment scores.
The median was calculated based upon the sequences showing the highest alignment scores (see Methods section).
Primers for RT-PCR were designed with Software OLIGO 4.0 based upon the sequences of the genes corresponding to the identified spots on the chip.
Based upon the sequence similarity searches, we found Vitis to have the greatest number of hits to contigs from both species.
The primers were designed based upon the sequences of the CcBV PTPs: each pair of primers is specific for each gene and enables the amplification of a DNA sequence encoding the 10 conserved motifs that characterize PTPs.
A phylogenetic reconstruction of the entire family, based upon the sequences of the ndhF and rbcL genes, partially confirmed previous results obtained by the analysis of the trnL-F and rps16 chloroplast regions [ 4].
Therefore, based upon the sequence identity between these genes, we designed a consensus oligonucleotide primer, T3con2, that would be suitable for the identification of the region containing the initiator methionine of the TACC3 cDNAs from primates and rodents.
An scFv that binds the external domain of EGFR, 2224, was synthesized by Life Technologies based upon the sequence provided in patent US 20100009390 A1 and subcloned into the pCT4Re constructs to create pCT4Re-2224 and pCT4Re-2224-intein.
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