All exposures were measured based upon subject self-reports, and some degree of exposure misclassification may have occurred.
Potential SA participants were identified using the Nam Pechan software based upon subject name and visual inspection by PSG [ 12]; and for AC subjects practice staff were consulted.
We estimated 3 parameters, based upon subject's performance, using the Hierarchical Gaussian Filtering Toolbox to compute maximum a posteriori estimates (Mathys et al. 2011; Mathys 2012).
This approach is based upon subjecting millions of pooled segregants to a selection for a phenotype and then analysing genome-wide changes in allele frequency within the population.
For treatment of a bleeding episode, the dose was based upon subject's PK profile, WFH guidelines and local standard of care, with a minimum dose of 25 IU kg−1 rIX-FP.
STI history was based upon subject self-reported history of the following infections: gonorrhoea, syphilis, genital herpes, genital warts or condylomas, human papillomavirus, chlamydia, HIV, AIDS, trichomonas, and/or any other sexually transmitted disease.
The hamster products were not sequenced, but based upon subject rankings derived from the experience with the canine products, 510 (46.9%) resulted in amplifications with a subjective ranking of 2, 3, or 4 that were judged to be likely to contain the correct product (see Fig. 1 for an explanation of the ranking system and Table S1 [Additional file 1] for individual rankings).
Hospital admission and mortality data based upon study subjects' usual place of residence were mapped to SLA 2006 boundaries.
