Covariates were evaluated based upon known relevance from clinical literature review and/or inspection of exploratory data analysis.
The selection of the covariates investigated was based upon their relevance to the clinical pharmacology profiling of ipilimumab.
- In addition to global goals, the selection of the modules or other questions to be included in MICS should also be based upon the relevance of the goal to be measured by the module/questions for the country.
Twenty-eight additional genes were also included in the siRNA library based upon genetic relevance to TNBC and elevated RNA expression in M6 cells and C3(1)/Tag tumors [see Additional file 1, Table S1].
Second, existing bioinformatics tools, including the IPA software (http://www.ingenuity.com/) and Endeavour (Aerts et al., 2006) were used to evaluate the potential impact of individual transcription factors based upon their relevance to bone biology.
The choice of covariates included in multiple regression models was based upon assessment of relevance and non-interference using directed acyclic graphs as previously suggested [ 24].
The tumor WES reports comprise an annotated listing of all somatic (tumor-specific) mutations identified in the patient's tumor, prioritized based upon potential clinical relevance for the patient-participant.
These SNPs were selected based upon previous citation/functional relevance in the literature, or a minor allele frequency of ≥ 10% among CEPH (Centre d'Etude du Polymorphisme Humain) Utah European ancestry samples (CEU) in HapMap and an ability to tag major haplotype blocks within each gene in the same samples [ 40].
Therefore, we assumed that the prior probability of a true association was increased if 1) the SNP was located within or near a known gene, and 2) if that locus had immune system relevance based upon public databases.
In addition to assessing the strength of methods, the relevance of the study was also assessed by setting limits for rates of application that would be considered environmentally relevant (based upon current recommended use rates [20]) and assigning a score of 0, 1, or 3 for relevance.
