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Alternatively, a more cost efficient option compared with buying a complete kit involves ordering a synthetic bait library, reamplifying this by PCR [ 24], optionally transcribing this into RNA and undertaking a do-it-yourself enrichment experiment based upon published protocols.
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Protocols for swim maze were based upon published methods for testing visual discrimination [ 38].
Fish were assigned trophic groups based upon published accounts of diet studies.
All oligonucleotide primer sets were designed based upon published mRNA sequence.
Each model parameter was assigned a unique probability distribution based upon published estimates of uncertainty.
Primers for RT-PCR were chosen based upon published breakpoint sequences.
The assay was performed based on published protocols (Andrews2001; Wiegand et al.2008) with slight modifications.
The method used to generate standard hEBs was based on published protocols [61].
Procedures for the manipulation of ES cells were based on published protocols [34].
Protocols used on this study were modified or newly designed based on published protocols [ 35, 44- 46].
The protocols for sample preparation and NMR acquisition are based on published protocols by Dona et al (14).
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