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Primers were constructed based upon previously reported sequences (Burnell et al. 1989; Ehrig et al. 1990; Mulligan et al. 2003; Osier et al. 1999; Yin et al. 1984).
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The initial set of conditions for the biochemical species (Supporting information Table S3) was decided based on upon previously reported values [7], [11] [12].
Some researchers made use of already existing test batteries (e.g. [ 15, 22, 23, 47, 48]), others adopted cognitive paradigms of related disciplines (e.g. [ 19, 33]) or based their choice upon previously reported effects in RF EMF research (e.g. [ 11, 12]).
A biotinylated Smac mimetic (2) was designed based upon our previously reported potent, conformationally constrained Smac mimetic (1).
Chemical structures of the antagonists are depicted in Additional file 2. Doses were based upon previously published reports [ 14, 19].
The protocol was based upon previously published IHC [ 40].
Autophagic vacuoles were identified based upon previously established criteria.
Enterocyte isolation was perfomed based upon previously published methods [ 20].
Solid and enzyme loading concentrations were based upon previously optimized experiments for dilute acid pretreatment [ 17].
The conceptual design of tools is based upon previously published work [ 10, 11].
Calculations are based on previously reported kinetic data.
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