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For the methylation analysis, the primers for the three Igf2/H19 DMRs were designed and based upon previously published sequences.
We performed multivariable logistic regression to determine predictors of willingness to participate, with predictor variables chosen a priori based upon previously published data or suspected influence on willingness to participate in research.
Rather, the aim of the research was to highlight the potential economic benefit that could result from the increase in human milk consumption on preterm infants based upon previously published odds ratios on specific outcomes depending on whether infants were fed formula or human milk.
This was expected, based upon previously published work showing gene-to-gene variability in expression patterns, even within monoallelically expressed gene clusters.
The method used to detect CD4 and CD8 T cell responsiveness by secretion of Interleukin-2 (IL-2) and/or IFN-γ to HIV peptides via ICS was based upon previously published methods [5].
Enterocyte isolation was perfomed based upon previously published methods [ 20].
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The α-FeOOH lattice parameters and initial atomic positions were based upon the previously published experimental X-ray diffraction measurements of Szytula et al. [13].
Based upon a previously published work showing that secreted Mtb Ndk manipulates the Rho GTPase regulatory cycle [26] and our recent observation that pathogenic mycobacteria express GAP activity towards Rab7 GTPase [17], we over-expressed and purified recombinant Mtb Ndk to homogeneity and examined its interaction with both Rab7, and the closely-related molecule, Rab5.
The structural model (Supplementary Figure S2) is based upon a previously published PBPK model.
The PBPK model developed herein was based upon a previously published model to which an infant submodel was added.
Based upon our previously published data [ 25, 26] and published reports [ 2- 12], we hypothesize that central clock mechanisms in the chick pineal gland are likely identical or, at least, very similar in the retina and must be retained in vitro.
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