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To get a better understanding of the biochemical processes in the growing shoot tips of grapevines affected by water deficit over time, a large number of detected proteins were displayed in newly constructed metabolic maps that were based upon previously generated maps in AraCyc [ 27], KEGG [ 28], and VitisNet [ 29].
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The sequencing results derived from bisulfite-PCR cloning demonstrated that both newly inserted Alu elements were indeed heavily methylated, as anticipated based upon our previously generated high-throughput bisulfite sequencing data (AI10 and AI11, Additional File 3, Figure S1).
Successive sets of solutions are generated depending upon previously generated sets.
The integration of lactic acid fermentation and Reverse Electro-Enhanced Dialysis (REED) is investigated based upon previously developed mathematical models.
For the methylation analysis, the primers for the three Igf2/H19 DMRs were designed and based upon previously published sequences.
Autophagic vacuoles were identified based upon previously established criteria.
The protocol was based upon previously published IHC [ 40].
Enterocyte isolation was perfomed based upon previously published methods [ 20].
Solid and enzyme loading concentrations were based upon previously optimized experiments for dilute acid pretreatment [ 17].
The conceptual design of tools is based upon previously published work [ 10, 11].
Chemical structures of the antagonists are depicted in Additional file 2. Doses were based upon previously published reports [ 14, 19].
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