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The design and synthesis of 12 were based upon previously established structural requirements for the stability of the 1,2,3,5-tetrazepin-4-one ring system.
Mitochondria were subclassified as either morphologically normal (class I), damaged (class II) or abnormally condensed/aggregated based upon previously established criteria ([59] and refer to Figure S10).
Autophagic vacuoles were identified based upon previously established criteria.
Sample size calculations are based upon the primary outcome measure compliance, and are based upon previously established compliance rates to the same program when advocated through written materials [ 12].
Similar(56)
We sought to find a difference in the mean quality of bowel preparation (based upon our previously established scale) of 1.0, and hypothesized based upon previous pilot experience developing and validating the scale that the standard deviation of this data would also be 1.0.
Samples were quantified based upon a previously established standard curve made up of known bacterial counts.
Samples were quantified to calculate the colony forming equivalent (CFE) based upon a previously established standard curve of bacterial colony forming units ranging from 1 × 10 to 10 cfu/mL.
For the methylation analysis, the primers for the three Igf2/H19 DMRs were designed and based upon previously published sequences.
The integration of lactic acid fermentation and Reverse Electro-Enhanced Dialysis (REED) is investigated based upon previously developed mathematical models.
The protocol was based upon previously published IHC [ 40].
Enterocyte isolation was perfomed based upon previously published methods [ 20].
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