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For the methylation analysis, the primers for the three Igf2/H19 DMRs were designed and based upon previously published sequences.
The integration of lactic acid fermentation and Reverse Electro-Enhanced Dialysis (REED) is investigated based upon previously developed mathematical models.
The protocol was based upon previously published IHC [ 40].
Autophagic vacuoles were identified based upon previously established criteria.
Enterocyte isolation was perfomed based upon previously published methods [ 20].
Solid and enzyme loading concentrations were based upon previously optimized experiments for dilute acid pretreatment [ 17].
The conceptual design of tools is based upon previously published work [ 10, 11].
Chemical structures of the antagonists are depicted in Additional file 2. Doses were based upon previously published reports [ 14, 19].
Sample size for the study was calculated based upon previously published data [ 37, 38] and Cohen's Power Primer [ 74].
Participatory methods used in this study were based upon previously published participatory and ethnobotanical study designs [ 27, 49, 50].
Third, migraine diagnosis was made based upon information collected using a well-established structured questionnaire [32,33].
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