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The article concludes with the description of an optimization environment for Diesel engine management based upon previously achieved emission models, and which is used in order to obtain an optimal performance regarding fuel consumption, emissions and drivability.
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For the methylation analysis, the primers for the three Igf2/H19 DMRs were designed and based upon previously published sequences.
The integration of lactic acid fermentation and Reverse Electro-Enhanced Dialysis (REED) is investigated based upon previously developed mathematical models.
The design and synthesis of 12 were based upon previously established structural requirements for the stability of the 1,2,3,5-tetrazepin-4-one ring system.
Autophagic vacuoles were identified based upon previously established criteria.
The protocol was based upon previously published IHC [ 40].
Enterocyte isolation was perfomed based upon previously published methods [ 20].
Solid and enzyme loading concentrations were based upon previously optimized experiments for dilute acid pretreatment [ 17].
The conceptual design of tools is based upon previously published work [ 10, 11].
Chemical structures of the antagonists are depicted in Additional file 2. Doses were based upon previously published reports [ 14, 19].
Participatory methods used in this study were based upon previously published participatory and ethnobotanical study designs [ 27, 49, 50].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com