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Based upon previously accrued information about the role of PGs in cancer and the currently available experimental evidences along this line, we have addressed the possibility that the pattern of expression of individual PGs, or groups of PGs, may act as either pro- or anti-tumourigenic and thereby be predictive, or indicative, of a discrete disease course in oral cavity HNSCC disease course.
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The integration of lactic acid fermentation and Reverse Electro-Enhanced Dialysis (REED) is investigated based upon previously developed mathematical models.
For the methylation analysis, the primers for the three Igf2/H19 DMRs were designed and based upon previously published sequences.
Mitochondria were subclassified as either morphologically normal (class I), damaged (class II) or abnormally condensed/aggregated based upon previously established criteria ([59] and refer to Figure S10).
Autophagic vacuoles were identified based upon previously established criteria.
The protocol was based upon previously published IHC [ 40].
Enterocyte isolation was perfomed based upon previously published methods [ 20].
Solid and enzyme loading concentrations were based upon previously optimized experiments for dilute acid pretreatment [ 17].
The conceptual design of tools is based upon previously published work [ 10, 11].
Participatory methods used in this study were based upon previously published participatory and ethnobotanical study designs [ 27, 49, 50].
Sample size for the study was calculated based upon previously published data [ 37, 38] and Cohen's Power Primer [ 74].
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