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This research introduces an optimal concurrent design approach based upon a previously developed distributed product development life-cycle modeling method.
The strategy used to construct the mutant strains is based upon a previously described method [21].
Based upon a previously published work showing that secreted Mtb Ndk manipulates the Rho GTPase regulatory cycle [26] and our recent observation that pathogenic mycobacteria express GAP activity towards Rab7 GTPase [17], we over-expressed and purified recombinant Mtb Ndk to homogeneity and examined its interaction with both Rab7, and the closely-related molecule, Rab5.
This instrument was based upon a previously piloted questionnaire [ 14].
Samples were quantified based upon a previously established standard curve made up of known bacterial counts.
The structural model (Supplementary Figure S2) is based upon a previously published PBPK model.
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Competitive RT PCR to quantify S100A4 mRNA was based upon a previously-described method (Taylor et al, 1998) with the following modifications.
The elements of the questionnaire that considered perceived adequacy of training were based upon a questionnaire previously developed by Thomas and colleagues [ 2].
Based upon LX2421, a previously identified antiplatelet aggregation agent, a series of novel 2-amino-3- naphth-2-yl propanoic acid derivatives were designed, synthesized and evaluated.
The WI-38 fibroblast scratch model was based upon a modification of a previously published protocol [33].
Scientists estimated this lessened risk based upon a lower likelihood than previously thought of slow shaking near New York City, which would be more likely to cause damage to taller structures from an earthquake in the vicinity of the city.
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