An omnibus likelihood ratio based test was performed to investigate whether the regression coefficient of at least one of the haplotypes in the model is not equal to zero.
T24 human bladder cancer cell line based test was performed to evaluate the chemopreventive/antiproliferative potential of EGCG and results showed that EGCG inhibits phosphatidylinositol 3′-kinase/Akt activation that in turn shows role in the modulation of Bcl-2 family proteins, leading to enhanced apoptosis of T24 cells [ 49].
Several physical performance-based tests were performed as well as assessments of sensory functions.
To evaluate patient outcome after treatment, performance-based tests were performed and self-reported questionnaires were filled in by participants at baseline and directly after treatment.
Gene-based tests were performed utilizing genome-wide association summary statistics results from the recent International Headache Genetics Consortium IHGCC) study comparing 5,118 MA cases to 74,239 controls, and 7,107 MO cases to 69,427 controls.
Gene-based tests were performed for each of the selected candidate genes, using the meta-analysis results of all SNPs tested in the respective genes (Table 3).
Therefore, we undertook a complementary approach, also using the WGS data from chromosome 3 in the UK10K project, where phenotypes were simulated under the null hypothesis and both single-marker and window-based tests were performed across the chromosome.
Gene-based tests were performed on the meta-analysis results using VEGAS, a program in which SNPs are assigned to genes, and their combined effect within a gene is tested taking into account SNP linkage disequilibrium (LD) (Liu et al. 2010).
Agarose gel based tests were also performed.
Regression based omnibus tests were performed for all haplotypes with >1% frequencies at a given locus.
