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Decreased sensitivity in oral tests compared to blood based tests has been reported [ 14] but there are limited available data on the performance of whole blood tests in the context of self-testing.
Although polymerase chain reaction-based tests have been developed, they are not widely used in areas where malaria is common as of 2012, due to their complexity.
Finite state machines based testing has been successfully applied to RBAC systems and enabled to obtain effective test cases, but very expensive.
Its development as a real time PCR based test has been reported [ 45].
Urine based testing has been successful for the detection of common sexually transmitted infections, including Chlamydia trachomatis and Neisseria gonorrhoea.
PCR based testing has been called into question, as false positives can be because of cross-reactivity with related viruses, or to laboratory contamination.
Now it is clear that recessive inheritance is also possible and mutations may have been missed in similar cases, because heteroduplex based testing has been used until recently in many large diagnostic centers.
VL based on the PERT assay, or on other RT-based tests, have been shown to correlate well with RNA-based VL over a wide dynamic range 10-100 cp/ml) [ 6- 8].
Antibody-based tests have been developed during the last decades.
In some cases criterion-based tests have been standardised on large representative populations.
The results of the parent study focusing on strength and functional performances as quantified by semi-objective (stopwatch) performance-based tests have been published previously [ 20].
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