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Genetic algorithm based strategy is used to obtain optimum orientation of the parts for RP process.
The internal model control based strategy is used to improve robustness for communication delays of the secondary level.
PCR based strategies were used for genotyping and the following PCR primers were used to detect Pik3c3- (deleted) allele: L1, 5'- AACTGGATCTGGGCCTATG-3'; L2 5'-GAAGCCTGGAACGAGAAGAG-3'; L3, 5'-CACTCACCTGCTGTGAAATG-3'.
Several evidence based strategies were used to maximise the response rate.
Study design: A population-based strategy was used to enroll children with CP at 6 geographic sites.
A mechanism-based strategy was used to design analogs that mimic the substrate or product of the reaction and form covalent interactions with the enzyme through the MIO prosthetic group.
STUDY DESIGN: A population-based strategy was used to enroll children with CP at 6 geographic sites.
Briefly, a PCR-based strategy was used to amplify 2 DNA amelogenin fragments (N-terminal and C-terminal) that could be ligated together.
An evidence-based strategy was used to follow-up patients at twelve months with postal questionnaires.
A non-covalent peptide-based strategy was used for efficient delivery of miR-122 mimic or inhibitor, which appeared to be effective to alter cholesterol levels without cytotoxicity.
A similar PCR-based strategy was used for production of full-length TNFα DNA templates except the reverse primer included a stop codon.
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