Exon-based sequencing of the SDH complex subunit genes SDHA-D identified no SDHX mutations, and the tumor was immunohistochemically positive for SDHB expression [ 20].
Population-based sequencing of the V3 region was carried out using the Big Dye® Terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA) with primers (3pmol) V3-for and V3-rev.
To see whether the two-copy structure is conserved in nonhuman primates, we first conducted PCR-based sequencing of the entire coding region of the 16 non-human primate species (table 1).
This is normally followed by 'bottom up' proteomic identification of the purified proteins, which entails proteolytic cleavage of the protein mixture (usually by trypsin) followed by MS-based sequencing of the resulting peptides, from which the protein identities can be deduced.
Only one of the seven insertions and deletions unique to the PacBio sequence is supported by alignment to additional strains, suggesting that the Illumina-based sequencing of the N16961 strain provided more sensitive and specific detection of insertions and deletions than the PacBio-based sequencing.
Our analysis of the Jindo dog genome using Illumina massively parallel sequencing technology is, to our knowledge, the first application of next-generation sequencing techniques to a dog genome since the traditional Sanger method-based sequencing of the boxer and poodle genomes.
All laboratories perform population-based sequencing of the full protease gene and at minimum codons 28 225 of the reverse transcriptase gene by means of commercial assays (Viroseq version 1, PE Biosystems; Virsoseq version 2, Abbott AG; vircoTYPE HIV-1 assay, Virco Lab) and in-house methods [ 9].
The four flashing colors would reveal the base sequence of each replica DNA, which would tell them the base sequence of the matching RNA from which it was derived.
The phylogenetic tree was generated through a molecular phylogenic analysis based on the 16S rRNA gene base sequences of the isolated strains.
