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Methods: Classical virus isolation approaches were compared with an RT-PCR based screening approach for the detection of influenza A virus in cloacal swabs and droppings collected from wild birds.
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A ligand based screening approach was used for the inhibitor design.
We established a novel adenovirus-free PCR based screening approach that amplifies tissue-targeted library viruses and therefore allows for multiple AAV library screening rounds after systemic application in vivo.
The target based screening approach involves purification of target protein, further compound screening assays against the target and in vivo target validation.
In this study public preferences for the screening of CRC were elicited to understand the potential of new biomarker based screening approaches.
Here we develop a reporter-based screening approach for high-throughput identification of chemical compounds that can modulate precise genome editing through homology-directed repair (HDR).
We developed and implemented a high-content, flow-cytometry-based screening approach for rapid, precise, and quantitative measurements of pharmaceutical control over autophagy.
Together, these results validate the yeast-based assay as an effective cell-based screening approach for compounds that interfere with EBV immune evasion and that might, therefore, provide treatments for EBV-related diseases, including cancer.
The work thus demonstrated the workability of in silico based screening coupled with a synthetic biology approach for gene synthesis for identification and production of a thermostable enzyme.
Two measures of information based on multigene expression profiles are considered for a backward information-driven screening approach for selecting important gene features.
Since currently no approaches have been published to carry out a broad screening approach for mono-ADP-ribosyltransferases, we optimized a novel method based on protein microarrays.
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