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LEP G2548A and LEPR 668A/G polymorphisms were analyzed by polymerase chain reaction based restriction fragment length polymorphism (PCR-based RFLP) methods.
PCR-RFLP, Polymerase Chain Reaction based – Restriction Fragment Length Polymorphism.
The MTHFR C677T mutation was analyzed by polymerase chain reaction (PCR) based restriction fragment length polymorphism (RFLP) methods.
MDR1 C3435T (rs1045642) polymorphism was analyzed by polymerase chain reaction (PCR)- based restriction fragment length polymorphisms [ 16].
One hundred thirty-three patients were genotyped for rs 3212227 SNP using polymerase chain reaction based restriction fragment length polymorphism (PCR-RFLP) analysis assay.
One-hundred-sixty-one patients were genotyped for rs1800795 and rs8192284 SNPs using polymerase chain reaction based restriction fragment length polymorphism (PCR-RFLP) analysis assay.
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VDR polymorphism was determined by polymerase chain reaction-based restriction fragment length polymorphism methods.
This study was performed by polymerase chain reaction-based restriction fragment length polymorphism (RF LP).
DNA was extracted from whole blood and genotyped for three markers in the TH-INS-IGF2 region by a polymerase chain reaction-based restriction fragment length polymorphism assay (PCR-RFLP).
They are easily detected in a quantitative way by real-time polymerase chain reaction (qRT-PCR) or microarrays and by other less frequently used identification methods, such as traditional northern blotting, PCR-based restriction fragment length polymorphisms (PCR-RLFP), ligation based measurement, and direct sequencing using next generation sequencing (NGS) platforms [ 55].
SNPs without length changes (base substitutions) were genotyped by a PCR-based restriction fragment length polymorphism (PCR-RFLP) method (primer information, restriction enzymes, pattern of polymorphism, and PCR conditions are in Table 6) [32], [32].
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