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"We're trying to build the web-based replacement of the local desktop as most of us know it today".

The mrc1::HIS3 was constructed by PCR-based replacement of the MRC1 ORF in BY4742 and the resulting strain was crossed with BY4741 tlc1::LEU2 to obtain heterozygous diploids.

The G418 resistant rad9, rad24, chk1, pds1 and dun1 mutants (Invitrogen) were crossed with the BY4741 tlc1::HIS3 mutant (constructed by PCR-based replacement of the TLC1 sequence) to obtain heterozygous diploids.

In addition, the diversity amongst the individuals is controlled by means of the proposed Affinity Based Replacement Strategy (ABRS) mechanism.

In order to check for the correct integration of PHLEO based replacement cassette phleo gene probe was used.

The method is based on replacement of the state register by a state counter.

In [17], a high capacity speech watermark was proposed based on replacement of the linear predictive (LP) residual with a watermark pulse.

In this paper, we introduce a method based on replacement of the unknown function by truncated series of the well-known shifted Chebyshev (of third-kind) expansion of functions.

The method is based on replacement of the non-linear vector field by a set of linear ones, each valid over a short segment of the evolving trajectory or over sufficiently small interval of time.

The design of the peptidomimetic compounds was based on the replacement of the intervening amino acid sequence of a Syk-binding di-phosphopeptide by non-peptide spacers based on either ethylene glycol or amino-propynyl-benzoate. Collision-induced dissociation (CID) spectra of the protonated molecular ions [M+H]+ allowed full characterisation of the peptide hybrids.

The main idea of the proposed technique is based on the replacement of the conventional CMOS inverter at the output of the logic gates with a new low-swing voltage inverter based on multithreshold voltage technology (LSIM).

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