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This dataset was generated using Massive Parallel Signature Sequencing (MPSS), a technique which involves the sequencing based quantification of over 2 million short ESTs per sample [ 36]. EST populations of nuclear and post-nuclear subcellular fractionations were examined in the male mouse cell line BLK CL.4 and male mouse liver tissue.
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It is clear that while further optimization of MS based quantification is beneficial, our current quantification of the stromal proteome using spectral counting does provide an excellent overview of over 550 stromal proteins, with relatively small variation and a dynamic range of four orders of magnitude.
This examination resulted in the identification and quantification of over 1300 unique ubiquitination sites.
The method is based on quantification of T-cell receptor excision circles (TRECs) extracted from DBSS.
As a result, a growing number of significant statements based on quantification can now be made.
First he outlined a formal logic based on allowing quantification over both elements and relations, and then he turned to a more detailed study of the quantifier-free formulas of this system that involved only relation variables.
Based on an absolute quantification of Ago proteins and total miRNA copies per cell, the authors come to the conclusion that there is a 13-fold excess of miRNA over Argonaute molecules in HeLa cells.
Quantification of MFI over 24 hours in the different treatment groups is shown in Figure 5.
Fig. 6 Divergent quantitative diagnosis based on method of quantification.
Quantifications were based on triplicates of three independent experiments.
Quantifications were based on duplicates of at least three independent experiments.
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