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RNA was then isolated from these spiked samples and applied to RT-qPCR and RT-ddPCR based quantification as described below.
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The delta Ct method was used for quantification as described in ref. 11 using GAPDH as the normalizer.
The surfaces were rinsed three times with PBS, followed by fibroblast adhesion and area quantification as described above.
Frozen disks were prepared for cAMP quantification as described [30].
Pellets were left to evaporate and solubilised in water for 2DOG quantification as described below.
The FITC-CBD quantification was performed as described elsewhere [ 25].
EPXMA, spectra processing and quantification were performed as described.
(E ) Quantification of the effect of U0126 treatment on CBFβ protein levels based on experiments as described in (A D ).
Quantification was performed exactly as described previously [ 22].
Based on the described staining protocols, similar reconstructions and quantifications as described for mouse liver tissue can be applied to human and pig livers.
Quantification of BCL11B mRNA was performed as described elsewhere [24].
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