We have demonstrated that RNA obtained from as few as 400 cells collected in a microneedle from the primary tumor, when amplified as cDNA using the PCR based protocol, can be used for microarray expression analysis.
Query-based protocols can be used for dynamic network topologies, and they also promote multiple route replies.
In general, virtual infrastructure-based protocols can be divided into (i) backbone-based approaches (e.g., [11]), and (ii) rendezvous-based approaches (e.g., [12]) depending on how the virtual infrastructure is formed by the set of potential storing nodes.
Arguably, however, if the histopathological evaluation is itself subject to errors then the estimates of the sensitivity and specificity of the light-based protocols can be expected to be biased.
Based on this assumption protocols can be used covering not the whole lung but sparing the lung bases to maximise the distance between the fetus and the edge of the image [65, 76].
Alternatively, the protocols can be based on elementary algorithms, either on paper or computerized, which allow less freedom for the nursing staff (14– 26).
Patient selection for these protocols can be based on the location of their metastatic deposits, preferring lung and skin, as well as on positive DTH to autologous melanoma cells.
