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Recently, a fragmentation-free PCR based protocol has been published, termed recombination-dependent PCR, which is easy to perform.
In summary, a new Suzuki Miyaura based protocol has been developed for accessing important 19-substituted geldanamycin Hsp90 inhibitors, compounds which we have previously shown to be significantly less toxic to normal endothelial and epithelial cell systems than their parent quinones and, as such, have considerable potential as therapeutic agents.
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In order to solve the problems, indirect electrochemical and biosensor based protocols have been often employed.
An imaging-based protocol has been applied to a high frequency time series (every second day between April 2003 to April 2004 on average) of zooplankton obtained in a coastal site of the NW Mediterranean Sea, Villefranche Bay.
The MLTC-based protocol has been applied to activate and expand in vitro circulating T cells from cutaneous melanoma patients.
Recently, BODIPY-flow cytometry-based protocol has been used to evaluate the lipid accumulation performance between wild-type and transgenic mutants of the diatom Thalassiosira pseudonana [ 22].
The original PEI-based protocol has been used in our laboratory successfully for transfections with HEK-293 (human embryonic kidney) and PC-12 (rat pheochromocytoma) cells.
Enzyme linked immunosorption assay based analytical protocol has been often referred for the selective detection of LAF in real samples.
A fast and reliable message system based on the SNMP protocol has been developed.
Magnetic resonance imaging based on the SSFP protocol has been suggested for the scans.
The modified protocol has been proposed based on ASGK with some modifications to balance the number of affected members and the encryption/decryption overhead with any number of the areas when a member joins or leaves the group.
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