Sentence examples for based protocol as described from inspiring English sources

After centrifugation, the cell pellets were lysed and prepared for RNA preparation and cDNA synthesis, with subsequent real-time PCR analysis using the SYBR-Green based protocol as described above.

Interestingly, this structure also required a combined chemical shift/NOE based protocol as described here.

DNA was extracted using a proteinase-K based protocol as described previously (Tanemura et al, 2009) and quantified using a Quant-iT PicoGreen dsDNA kit (Molecular Probes, Eugene, OR, USA).

RNA was extracted using both chloroform and column based protocols as described in Additional file 2. Quality and quantity of the extracted RNA was verity before storage at −80°C.

Real-time PCR of MCP-1 was performed following the SYBRGreen PCR based protocol as previously described [11].

All participants followed the same protocol, as described elsewhere (20).

R. conorii were isolated using a bead-based protocol as described previously (11).

Positive controls were generated by a primer-mediated PCR mutagenesis-based protocol as described (Ruiz et al, 1997).

Genomic DNA from C. violaceum ATCC 12472 was isolated using a CTAB-based protocol as described previously [ 72].

Total RNA isolation and double stranded cDNA library preparation was done by PCR-based protocol as described previously (Dixit et al., 2009).

For the construction of reporter plasmids containing substitution mutations in factor binding sites of c-Myc or Sp1, site-specific mutagenesis was performed by a PCR-based protocol as described previously (Kyo et al, 2000).