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As the reliability of the phylogeny output is strongly dependent upon the quality of the starting multiple sequence alignment, and the use of constraints derived from structural data has been shown to increase the accuracy of sequence alignments [31], we based our alignment of the TFP sequences using information from the multiple superposition of TFP 3D structures.
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Based on our alignments of complete gene sequences in bats, we identified two highly variable regions (exons 7 and 17).
We performed two separate analyses of our conserved clusters, one based on alignment of nucleotide sequences and another based on alignment of predicted peptide sequences.
Ideal positioning for each implant was determined based on alignment of the implant neck and head within the original bone (Fig. 1).
Whereas the cladogram in Fig. 1 is based on alignment of proteins, we used gene names for clarity.
The most 5' primer was also based on alignment of multiple copies of the A repeat from Mus musculus and three Microtus species [7], [10].
PCR was performed on the RNA with primers to amplify the entire HA and NA genes (designed based on alignment of several H1N1 viruses).
Structure based sequence alignment of GGT proteins.
The structure based sequence alignment of AspRS from three different organisms is shown in Figure 4.
Furthermore, a taxonomic analysis based on alignment of core genomes was performed.
Song et al. [ 36] transferred functions based on alignment of domain content.
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