Thus, identification of different types of DEC includes biochemical reactions, serotyping, phenotypic assays based on virulence characteristics and molecular detection methods[ 7].
A spore suspension of C. gleosporoides in sterile distilled water was prepared from 7-10 days old single-spore cultures (based on virulence on D. alata) and standardised to 106 spores per ml with tween 80 (Merck)(1.2% v/v) added as wetting agent.
The dendrogram based on virulence data on differentials grouped the isolates into three major Clusters.
Based on virulence factor gene PCR, 23.2% of the strains were classified as ExPEC.
The clustering analyses based on virulence data on various taxonomic groups revealed differences between the icipe's isolates.
The AMDV genome is highly variable, and previous analyses of the virus in Europe suggest that strains can be divided into three main lineages, which do not appear to be based on virulence, geographic origin, or year of isolation (Knuuttila et al. 2009; Olofsson et al. 1999; Schuierer et al. 1997).
Finally, we introduce a new epidemiologic model for NTM disease based on virulence-exposure-host factors.
The factors predisposing one to infection are not well described, but likely result from interaction between host defense mechanisms and the load of exposure [ 13]. Figure 1 illustrates our proposed epidemiologic model for NTM disease based on virulence-exposure-host factors.
Once the putative virulence genes are identified in each user-defined strain, PathoProT will cluster (agglomerative hierarchical cluster analysis) the virulence genes and strains based on their virulence gene profiles and visualize them as a heat map with dendrograms.
We have shown that the strains, although showing some heterogeneity, could be classified into two groups, based on their virulence gene and GI content.
