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We sought to establish design principles for this class of biosensor by characterizing over 400 biosensors based on various protein analytes, binding proteins, and fluorophores.
Therefore, we set forth to explore machine-learning based methods based on various protein features for the prediction of CDKI proteins.
The FFAS03 server provided a ranked list of structural threading models based on various Protein Data Bank (PDB) entries; invariably the highest scoring models were based on PrP and Dpl structures [25], [62], [63].
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A series of engineered helper phages were constructed to facilitate both display valency and formats, based on various phage coat proteins.
This approach estimates a quantitative measure for the PFM similarity of two proteins, based on various features derived from their protein sequences.
As of today, several efficient systems of genome manipulation have been described based on various classes of DNA-binding chimeric proteins such us zinc-finger proteins (ZFs), transcription activator-like effectors (TALEs) and the guide RNA (gRNA -driven Cas9d mutant (CRISPR) system.
Brunak et al. applied modern machine learning techniques such as artificial neural networks or support vector machines to predict protein annotations based on various features derived from annotated amino acid sequences [19], [20].
We typified teleost fish class II genes and their protein products based on various classical/non-classical characteristics and then examined the possession of functional residues critical for the DM system.
The main advantage of our feature extraction methods over existing techniques is that features are extracted from the full length protein sequence based on various coding schemes without referencing external databases.
Since almost no tools are available for molecular biology experiments on Chlamydiales, a putative regulation of these lgr genes and a possible role of these large proteins are proposed, based on various in silico analyses.
We have investigated the organism specific recognition of protein-RNA complexes based on various sequence and structure based features such as binding propensity, preference of residues at conserved positions, binding segments, binding motifs, neighboring residues and interacting amino acid-nucleotide pairs.
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