This indicates, the purification strategy of protein M is entirely different to traditional IBPs and the salient purification feature of protein M would be a breakthrough for purifying not only non-mammalian antibodies, but also monoclonal antibodies and engineered antibodies based on variable region.
Phylogenetic trees based on variable region amino acid sequences of light (VK) and heavy (VH) chains of the 15 VEGF neutralizing RabMAbs are shown in Figure 2A, B. Three of the 4 human VEGF specific antibodies (EBV301, EBV304, and EBV308) are clustered in both the heavy and light chain lineages (group 1 in Figure 2A, B).
A set of oligonucleotide probes were designed based on variable regions of the 16S gene of the Escherichia coli, Proteus mirabilis, Klebsiella oxytoca, and Pseudomonas aeruginosa.
Based on variable sequence regions, specific primers were constructed for the differentiation of five Eimeria species (Eimeria acervulina, E. brunette, E. maxima, E. necatrix, and E. tenella).
Moreover, based on variable importance for the RF-model, we observed that wind erosion, technology development, aridity index, slope index, vegetation state and land-use change variables are relatively most important on desertification hazard of Taybad-Bakharz region, respectively.
Data on variables were measured based on variable description and variable type [see Additional file 2].
For risk adjustment, propensity scores were estimated for long term use and late use in separate logistic regression models based on variables including age, geographic region, race, diagnoses of pain conditions, calendar year of delivery, and additional risk factors for neonatal abstinence syndrome.
Species-specific oligonucleotide probes (S-SONP) for canine taeniid cestodes were then designed manually based on the variable region between the conserved primers.
Here, we describe the production of a recombinant antibody fragment based on the variable region of the SC142 monoclonal antibody using expression cloning without antigen.
To determine the reproducibility of the T20 score distinguishing most human and mouse sequences based on their variable region sequences, an independent set of human and mouse antibodies were evaluated.
A second generation DNA microarray could be developed for this purpose based on the variable regions identified between a number of sequenced strains, and utilise printed rather than spotted microarrays.
