For this study, treatment response is defined as a symptom reduction of the baseline BAI symptom severity score of at least 30%% and remission a score reduction of at least 30%% plus a total score <11, based on validation in The Netherlands Study of Depression and Anxiety (NESDA) [ 35– 35].
Ultimate evaluation of the method should be based on validation.
The confidence scores of this channel are based on the antibody validation scores provided by HPA (15) (see Methods section).
The ROC curves of the external validation, based on predicted scores, are shown in Electronic Supplementary Material Fig. S2.
It has been argued that these values for minimum clinically important difference may be too low in some contexts [ 113], hence we will repeat this analysis using a threshold of 50% reduction in Oswestry scores and NRS pain scores based on empirical validation studies suggesting that this may be a more suitable threshold for important differences [ 67, 114].
For the induction screen of the Kinase Inhibitor Library we selected hits for further validation based on z-score, which accounted for 21 compounds in total.
This is indeed possible but the number of validated peptides is lower than when performing validation based on score, differences between forward and a reverse database searches, and best and second best peptide matches, together with the scored peak intensity percent.
For tuber profiling, each sample was assayed twice in a two-colour dye experiment, allowing cross-validation of the observed variation and subsequent filtering based on correlation scores (Methods).
Based on our validation study, an AUDIT score of ≥8 had a sensitivity of 83.5% in this population, compared to a diagnosis of alcohol abuse or dependence based on the Composite International Diagnostic Interview (CIDI).
With great satisfaction, we could confirm that their referral –even not based on a sum score resulting from the KINDEX – was accurate based on the scores later calculated: a) the KINDEX sum score and b) the three global scores in the validation interview.
