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The assay uses primers WG-OLS-F1 and WG-OLS-R1 and the fluorescent probe WG-OLS-P1, designed based on unique sequences found only in the genome of oleander strain Ann1.
The reason that Overgo hybridization strategy was successfully used in the construction of integrated genetic and physical maps was that Overgo probes were designed based on unique sequences from the genome sequences, not from repetitive sequences.
A: Calculation based on unique sequences.
The majority of primers tested were based on unique sequences in the data set and amplified tri- or tetranucleotide repeats.
SEPT9_v1 and SEPT9_v3 isoform primers were either as published [ 21] or designed by hand based on unique sequences at the 5' end.
Locus specific primers were designed based on unique sequences flanking gene PGSC0003DMG400010146, which were used to generate and sequence an amplicon from the individuals of the CHIPS-ALL population.
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SRPKs represent a highly distinctive family of protein kinases based on unique sequence elements outside the conserved kinase domain.
Based on unique sRNA sequences mapped to miRBase, release 20.0 [ 22], with perfect matches and a minimum of 10 read counts, we identified 123 unique sequences belonging to 23 conserved miRNA families in TC7d and TD7d libraries, with total abundances of 90,472 and 137,058 reads per million (RPM), respectively (Table 2).
The forward and reverse primers were designed based on unique flanking sequences using Primer 3 (http://primer3.sourceforge.net/).net/
The 70 nucleotide probes were based on unique cDNA sequences from white spruce (P. glauca) [ 13] and Sitka spruces (P. sitchensis) [ 14] (Table 1).
Over 19,000 of the 20K microarray's 50-mer oligonucleotide probes were designed based on unique candidate sequences arising from the assembly of over 150,000 ESTs from 42 cDNA libraries [23 normalized, 19 suppression subtractive hybridization (SSH)] representing various tissues, treatments, and life stages (including over 20,000 ESTs from a normalized larval cDNA library) [ 4, 19].
More suggestions(15)
based on partial sequences
based on concatenated sequences
based on unique properties
based on unique traits
based on orthologous sequences
based on unique visitors
based on unique serializations
based on chaotic sequences
based on unique loci
based on centralized sequences
based on unique regions
based on unique identifiers
based on nucleotide sequences
based on unique peptides
based on observed sequences
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