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Primers specific for each receptor were designed based on unique exons (except for Dappu_InR4) from predicted cDNA sequences from FleaBase.
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Using this paradigm as a starting point, we identified three types of WC1 genes based on unique exon-intron structures.
We calculated gene expression level based on unique read counts and RPKM values (reads per kilobase of exon model per million mapped reads) for each contig and ESTs (the length of the ESTs was the exon length for the contigs).
Generally, lucrative opportunities also can surface based on unique circumstances.
A: Calculation based on unique sequences.
*OD=odds ratio based on unique family sets.
The exon estimates were computed based on all unique exons found in the genome.
Probes were designed based on the unique exon junctions formed by fusion events.
The ratios were first log-transformed (base 2) and then the transformed ratios were grouped based on annotated exons.
We calculated the density of available Mi{ET1} insertions in exons (24.9 inserts per Mb, based on 724 unique integration sites) and introns (20.7 insertions per Mb, based on 917 unique integration sites).
By this method, 52 novel retrocopies were called based on exon-exon junction data alone.
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