Sentence examples for based on unique barcodes from inspiring English sources
In total, 231,519 sequences, which passed the quality filters and were successfully assigned to a sample based on unique barcodes, were generated from these samples.
The raw sequences were sorted based on unique barcodes, trimmed for sequence quality, and clustered at 97 % identity for OTUs with USEARCH v7.0 (http://www.drive5.com/usearch/download.html) using UPARSE pipeline [ 46].
Paired-end reads were assigned to samples based on their unique barcodes and truncated by cutting off the barcode and primer sequence.
All sequences were analyzed with QIIME Pipeline Version 1.7.0, and sorted to each sample based on their unique barcodes.
Sequences were assigned to individuals based on their unique barcodes and filtered as described above.
We used two biological replicates for our analyses and computationally de-multiplexed the data by transposon construct based on their unique barcode.
(1) Sample demultiplexing: the multiplexed reads are assigned to samples based on their unique nucleotide barcodes in the mapping file.
The remaining sequences were assigned sample aliquots based on their unique nucleotide barcodes, including error-correction.
Other scientists have developed tracking systems based on genetic barcodes.
The method is based on DNA barcodes, i.e. standard regions of DNA, and high-resolution melting of amplicons.
