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Genes were named based on the best BLASTx result to mouse when the Expect value <1 × e−5, or based on transcript number if no homology was found.
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Irrespective of the pre- or post-molt stage, this newly identified protein had the highest gene expression compared to all other putative cement proteins located in the sub-mantle tissue based on transcript numbers (Fig. 3).
For comparisons with gene lists generated from the honey bee arrays (#3-5), it was possible to directly compare gene lists based on transcript AM numbers.
Since Medicago GeneChips are based on transcript and genomic sequences, the number of probe sets exceeds the number of genes represented.
Both numbers, 24'455 based on transcript redundancy or 28'520 based on genetic loci exceed the number previously predicted to be ~20'000 [ 3].
Numbers enclosed in parenthesis represent rank based on transcript frequency for the top 10 most abundant protein-coding transcripts in each library.
Third listening: Read out the story based on transcript without any pause.
Based on the transcript number, approximately a quarter of F. hepatica transcriptome (26.56%) showed homology with the sequences from helminth secretome database (HSD; Table 1a), but only a minor part of these transcripts (3.1%; n = 1,251) were out of the category of NVTs that these transcripts were named HTs (E value < 10−5; Table 1b).
A rough estimate of the number of Hydra genes based on transcripts only was deduced from the number of sequences in each dataset associated with a measure of the redundancies they contain.
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