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PASA was then again utilized to update this initial genome annotation from EVM and MAKER2 to add alternate splicing and UTR sequences based on transcript data [ 4, 5, 22- 26, 26].
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This calculation was based on transcripts data downloaded from the Ensembl Genes 74 database for Galgal4, from which multiple transcripts per gene were excluded.
On the one hand, prediction of AS based on transcript sequence data seems to be overestimated in several filamentous fungi as cDNA-derived sequence data without supporting protein sequence data do not allow discrimination between AS and unprocessed or partially processed transcripts.
Considering that RNA-Seq mainly estimates exon expression and that most genes have multiple transcripts, it is necessary to determine how to estimate gene expression level based on transcript expression data.
Up to now, information is based only on transcript data.
We also applied correlation network analysis based on transcript-metabolite data integration.
This research group also worked on modeling the transcriptome, based on transcript-sampling data (Zhu et al., 2008).
This tool has been successfully applied to model gene regulatory networks based on transcript profiling time series data of globally perturbed organisms [ 6].
Third listening: Read out the story based on transcript without any pause.
For 6p12.1, based on the transcript data, the breakpoint should be outside and proximal to ELOVL5.
Thus, we created a new chip definition based on recent transcript data.
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