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Based on transcript alignments, the PASA pipeline was used to correct the consensus gene model structure and to resolve conflicts between different isoforms.
GLEAN uses transcript alignments to support splice predictions, but does not merge or split genes based on transcript alignments, while MAKER2 creates gene models that best agree with transcript alignments, and works best with highly reliable transcript assemblies.
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The results demonstrated that TEVGs under dynamic flow conditions had better outcome than static culture controls in terms of cell proliferation and alignment, ECM production and cell phenotype based on transcript and protein level assessments.
Initially, the majority of C. elegans gene structure changes were based on the alignments of transcript data from large-scale transcriptome projects such as Yugi Kohara's EST libraries (Kohara,Y., unpublished data) and the ORFeome project (13).
In B. rapa, cis-NATs with more than 2 copies would crosstalk with each other and form trans-NATs. Based on pairwise alignments of transcripts, 47 cis-NATs that produce nat-siRNAs in B. rapa are conserved in Arabidopsis, implying that the two thirds of the cis-NATs in B. rapa generate specific nat-siRNAs.
We present a genome-wide comparative study of AS in 23 informative fungi of different taxa, based on alignments of public transcript sequences.
The latter case would require scaffolding to resolve based on alignments to reference transcripts, but was not conducted in these assemblies.
We then constructed multiple alignments of peptide sequences using ClustalW and constructed nucleotide alignments based on the peptide alignments.
Based on alignments by a rice transcript, 1,235 (39%) and 1,221 (43%) of non-TE-related genes on chromosomes 11 and 12, respectively, were active (Additional figure 10 [see Additional file 1]).
Transcripts were annotated with protein functional descriptions based on aggregate alignments with proteins from the non-redundant protein database maintained at NCBI (protein NR, downloaded from http://www.ncbi.nih.gov).nih.gov
Heber et al. (2002) first defined and identified splicing graphs based on EST alignments, but did not devise a method to obtain transcripts from the graphs.
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