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In addition, functional interpretations based on transcript abundance cannot distinguish between mRNA transcription vs degradation rates.
The primers sequences for the genes selected based on transcript abundance are shown in Additional file 6: Table S3.
Based on transcript abundance, 10 genes were identified with significant expression fold changes among the parental lines.
Therefore, we are not able to determine the extent of glyphosate metabolism based on transcript abundance in leafy spurge.
Using a transcript count of 32 as a lower bound, we were able to define a threshold for miRNA expression based on transcript abundance.
The following analysis was based on transcript abundance, therefore, Affymetrix probe-sets were used to represent genes and not AGI identifiers.
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The first approach is based on transcript inference and abundance estimation of the transcripts, as performed by tools like Cufflinks (Trapnell et al., 2010), rQuant (Bohnert and Rätsch, 2010), Trans-Abyss (Robertson et al., 2010) and Scripture (Guttman et al., 2010).
For example, the GmHK genes, which express more highly in roots than in shoots, can be divided into three groups (low, medium and high) based on their transcript abundance, in which the lowest (GmHK08) and highest (GmERS2) transcript abundance in roots was more than 3200-fold diFigrent (Fig. 1A).
Based on transcript and proteome profiling of developing xylem in Populus, Kalluri et al. reported that Potri.014G089400 had enhanced EST expression and protein abundance in xylem tissue [ 29].
It enables the precise elucidation of transcripts present within a particular sample, and can be used to calculate gene expression based on absolute transcript abundance [ 30].
Considering on FPGS expression based on the transcript abundance, FPGS Os10g35940 increased approximately twofold in the knockout leaf suggesting the compensation of FPGS activity from FPGS Os10g35940 during the vegetative stage with no changing of the leaf phenotype.
More suggestions(16)
based on transcript sequence
based on miRNA abundance
based on fractional abundance
based on transcript frequency
based on mRNA abundance
based on transcript analysis
based on bandwidth abundance
based on transcript level
based on fossil abundance
based on transcript information
based on transcript response
based on total abundance
based on transcript length
based on crustal abundance
based on genomic abundance
based on transcript annotation
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