Therefore, a strategy to determine the physiological functions and interactions of transcription factors based on the analysis of TFA profiles would be more fundamentally sound than one based on transcript levels per se.
Positive ones were screened for err1 expression based on transcript analysis, and the one with the highest increase in err1 expression compared to its parental strain (named PEC1) was chosen for further characterization.
In addition, functional interpretations based on transcript abundance cannot distinguish between mRNA transcription vs degradation rates.
Grün et al. [76] developed RaceID, an algorithm for the identification of rare and abundant cell types based on transcript counts obtained with UMIs.
The results demonstrated that TEVGs under dynamic flow conditions had better outcome than static culture controls in terms of cell proliferation and alignment, ECM production and cell phenotype based on transcript and protein level assessments.
Previous work suggested roles for Drosophila Importin-α2 in the nervous system based on transcript expression [21].
Based on transcript response profiles, only members of groups I, III, IV and VII remained differentially expressed compared to control at the end of the acclimation time-period.
PCR primers were designed within the introns of VEGF based on transcript NM_003376.3; see Table S3 for PCR primer sequences and figure 1 for the location of the primers.
The reason why we focus on co-regulation rather than regulatory interactions is that the values of the inference are based on transcript levels, and TFs are known to be expressed on a low level.
The reduction in transcripts for the IIA subunit cannot explain the degree of loss of clustering we see at the neuromuscular junction, arguing the problem in the SMN depleted tissue is not primarily based on transcript reduction.
Dror et al., based on transcript levels, reported a 10-fold excess in the number of cohesins available on the anchor proteins for attaching the scaffoldin CipA to the cell surface under conditions of low growth rate [35].
