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We constructed an ML tree for DRB alleles, including HLA-DRB1/ 3/ 4/ 5, Patr-DRB1, Mamu-DRB1, and Mafa-DRB1, based on total substitutions in only non-PBR nucleotide sequences.
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InParanoid analyses identified 758 4-taxa orthologs (ABCD), an additional 645 3-taxa orthologs (BCD) and 3437 ortholog groups with sequences from only clades C and D. After filtering based on total tree length (# substitutions per codon), 611 ortholog groups were analyzed with all 4 taxa represented (average tree length = 4.1) and 528 were analyzed with 3 taxa (average tree length = 2.5).
We wanted households to consider WTP based on total health care costs although we did not specify or point to a possible substitution of providers.
*Percentages based on total state population.
The MP analysis based on equally weighted total substitutions for combined data of 12S rRNA and cytochrome b sequences produced a tree with 893 steps, CI = 0.7828, HI = 0.2172, and RI = 0.9038.
A total of 17 genotypes (alleles) were recognized based on 9 substitutions in the sequence alignment (688 bp).
In total, 31 haplotypes (T1 T31), ranging from 309 bp to 314 bp, were designated based on 39 substitutions and three small (≤ 5 bp) indels (Additional file 4: Table S4).
We compare designs based on column substitutions and designs based on permutations.
The most widely used clocks based on synonymous substitutions aggregate many different types of synonymous substitutions.
However, substitution rates vary across the genome and between species that makes direct comparisons solely based on nonsynonymous substitutions difficult.
In this case, the evolutionary distance based on synonymous substitutions is expected to be greater than the distance based on nonsynonymous substitutions.
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CEO of Professional Science Editing for Scientists @ prosciediting.com