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Estimates of nucleotide diversity were based on total sequences and silent sites separately using DnaSP version 5.10.01 (Librado and Rozas 2009).
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The species richness analysis was performed by using MEGAN based on total sequencing reads [ 70].
Tags with a frequency of 1 were not considered for quantitative purposes, because these are likely to represent artifacts of sequencing or of the SAGE procedure [ 26]. a Calculation of the frequency distributon of a given tag was based on total tags sequenced in library.
clones] 0 [0] 100 [7] 100 [12] 100 [31] aPercentage based on total number of sequences for each retting period.
₤Percentage based on total number of high quality sequences.
Three chloroplast contigs were identified, with greatest similarity to Platanus occidentalis based on total alignment score and percentage sequence identity.
Gene coverage for each lily and tulip genotype was calculated based on total number of bases generated (assembled sequences and singletons) as a percentage of the assumed gene space (82 Mb).
We constructed an ML tree for DRB alleles, including HLA-DRB1/ 3/ 4/ 5, Patr-DRB1, Mamu-DRB1, and Mafa-DRB1, based on total substitutions in only non-PBR nucleotide sequences.
§Percentage based on total unique transcripts (782) with significant similarity to sequences in database.
The sequencing depth was estimated based on total length of the reads used in the assembly divided by total size of transcriptome assemblies.
The study was based on sequencing total DNA using either the 100 or 150-bp paired-end chemistry and Illumina HiSeq 2000, assembled using the polar bear genome sequence as a reference (Li et al. 2011).
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